Restriction Digest of Plasmid DNA

1.  Make a master mix of reaction

d2H2O 6.25 ul
Enzyme Buffer         1.5ul
BSA                       0.75ul
Enzyme(s)              0.75ul each or 1.5ul of a single enzyme

                            Add 10ul of this to a tube +

2. Add DNA (plasmid): 5-8 ul miniprep or 2ul midi or maxiprep

3. 37C incubation for 2h to overnight

4. Run 1%agarose in 1x TAE gel